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這是描述信息

ATP1A1

<p>Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)</p>

Elisa
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
Figure 2:Western blot analysis using ATP1A1 mAb against human ATP1A1 (AA: 153-288) recombinant protein. (Expected MW is 40.5 kDa)
Western Blot
Figure 3:Western blot analysis using ATP1A1 mAb against HEK293-6e (1) and ATP1A1 (AA: 153-288)-hIgGFc transfected HEK293-6e (2) cell lysate.
Western Blot
Figure 4:Western blot analysis using ATP1A1 mouse mAb against Hela (1), HepG2 (2), and A431 (3) cell lysate.
Flow cytometric
Figure 6:Flow cytometric analysis of Hela cells using ATP1A1 mouse mAb (green) and negative control (red).
Immunohistochemical analysis
Figure 5:Immunofluorescence analysis of Hela cells using ATP1A1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Immunohistochemical analysis
Figure 7:Immunohistochemical analysis of paraffin-embedded lung cancer tissues using ATP1A1 mouse mAb with DAB staining.
Immunohistochemical analysis
Figure 8:Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using ATP1A1 mouse mAb with DAB staining.

ATP1A1

貨號 : 32106

規格/濃度 : 1.0mg/ml

價格：

￥ 2557.5

瀏覽量:

1

說明書:

產品規范

Entrez GeneID	476
別名	CMT2DD; HOMGSMR2
克隆編號	3C1F3
分子量	112.8kDa
同種型	Mouse IgG2a
存放說明	4℃; -20℃ for long term storage
種屬反應性	Human;
免疫原	Purified recombinant fragment of human ATP1A1 (AA: 153-288) expressed in E. Coli.
存儲溶液	Purified antibody in PBS with 0.05% sodium azide

經測試應用

WB	1/500 - 1/2000
IHC	1/200 - 1/1000
ICC	1/200 - 1/1000
FCM	1/200 - 1/400
ELISA	1/10000

描述

The protein encoded by this gene belongs to the family of P-type cation transport ATPases, and to the subfamily of Na+/K+ -ATPases. Na+/K+ -ATPase is an integral membrane protein responsible for establishing and maintaining the electrochemical gradients of Na and K ions across the plasma membrane. These gradients are essential for osmoregulation, for sodium-coupled transport of a variety of organic and inorganic molecules, and for electrical excitability of nerve and muscle. This enzyme is composed of two subunits, a large catalytic subunit (alpha) and a smaller glycoprotein subunit (beta). The catalytic subunit of Na+/K+ -ATPase is encoded by multiple genes. This gene encodes an alpha 1 subunit. Multiple transcript variants encoding different isoforms have been found for this gene.

文獻

1.Autophagy. 2018;14(8):1359-1375.
2.Sci Rep. 2016 Jun 14;6:27738.

序列

153-288

圖表

Elisa

Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

Western Blot

Figure 2:Western blot analysis using ATP1A1 mAb against human ATP1A1 (AA: 153-288) recombinant protein. (Expected MW is 40.5 kDa)

Western Blot

Figure 3:Western blot analysis using ATP1A1 mAb against HEK293-6e (1) and ATP1A1 (AA: 153-288)-hIgGFc transfected HEK293-6e (2) cell lysate.

Western Blot

Figure 4:Western blot analysis using ATP1A1 mouse mAb against Hela (1), HepG2 (2), and A431 (3) cell lysate.

Flow cytometric

Figure 6:Flow cytometric analysis of Hela cells using ATP1A1 mouse mAb (green) and negative control (red).

Immunohistochemical analysis

Figure 5:Immunofluorescence analysis of Hela cells using ATP1A1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)

Immunohistochemical analysis

Figure 7:Immunohistochemical analysis of paraffin-embedded lung cancer tissues using ATP1A1 mouse mAb with DAB staining.

Immunohistochemical analysis

Figure 8:Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using ATP1A1 mouse mAb with DAB staining.

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