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這是描述信息

BRAF

<p>Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)</p>

Elisa
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
Figure 2:Western blot analysis using BRAF mAb against human BRAF (AA: 299-447) recombinant protein. (Expected MW is 46kDa)
Western Blot
Figure 3:Western blot analysis using BRAF mouse mAb against Hela (1), HT-29 (2), MOLT4 (3) T47D (4) HePG2 (5) HL-60 (6) and PC-12 (7) cell lysate.
Flow cytometric
Figure 4:Flow cytometric analysis of BEL-7402 cells using BRAF mouse mAb (green) and negative control (red).
Immunohistochemical analysis
Figure 5:Immunohistochemical analysis of paraffin-embedded bladder cancer tissues using BRAF mouse mAb with DAB staining.
Immunohistochemical analysis
Figure 6:Immunohistochemical analysis of paraffin-embedded cerebellar tissues using BRAF mouse mAb with DAB staining.

BRAF

貨號 : 32150

規格/濃度 : 1.0mg/ml

價格：

￥ 2557.5

瀏覽量:

1

說明書:

產品規范

Entrez GeneID	673
別名	NS7; B-raf; BRAF1; RAFB1; B-RAF1
克隆編號	2A10D7
分子量	84.4kda
同種型	Mouse IgG1
存放說明	4℃; -20℃ for long term storage
種屬反應性	Human; Rat;
免疫原	Purified recombinant fragment of human BRAF (AA: 299-447) expressed in HEK293-6e.
存儲溶液	Purified antibody in PBS with 0.05% sodium azide

經測試應用

WB	1/500 - 1/2000
IHC	1/200 - 1/1000
FCM	1/200 - 1/400
ELISA	1/10000

描述

This gene encodes a protein belonging to the RAF family of serine/threonine protein kinases. This protein plays a role in regulating the MAP kinase/ERK signaling pathway, which affects cell division, differentiation, and secretion. Mutations in this gene, most commonly the V600E mutation, are the most frequently identified cancer-causing mutations in melanoma, and have been identified in various other cancers as well, including non-Hodgkin lymphoma, colorectal cancer, thyroid carcinoma, non-small cell lung carcinoma, hairy cell leukemia and adenocarcinoma of lung. Mutations in this gene are also associated with cardiofaciocutaneous, Noonan, and Costello syndromes, which exhibit overlapping phenotypes. A pseudogene of this gene has been identified on the X chromosome. [provided by RefSeq, Aug 2017]

文獻

1，Proc Natl Acad Sci U S A. 2020 Dec 8;117(49):31105-31113.
2，Sci Rep. 2020 Oct 9;10(1):16943.

序列

299-447

圖表

Elisa

Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

Western Blot

Figure 2:Western blot analysis using BRAF mAb against human BRAF (AA: 299-447) recombinant protein. (Expected MW is 46kDa)

Western Blot

Figure 3:Western blot analysis using BRAF mouse mAb against Hela (1), HT-29 (2), MOLT4 (3) T47D (4) HePG2 (5) HL-60 (6) and PC-12 (7) cell lysate.

Flow cytometric

Figure 4:Flow cytometric analysis of BEL-7402 cells using BRAF mouse mAb (green) and negative control (red).

Immunohistochemical analysis

Figure 5:Immunohistochemical analysis of paraffin-embedded bladder cancer tissues using BRAF mouse mAb with DAB staining.

Immunohistochemical analysis

Figure 6:Immunohistochemical analysis of paraffin-embedded cerebellar tissues using BRAF mouse mAb with DAB staining.

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