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這是描述信息

HLA-F

<p>Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)</p>

Elisa
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
Figure 2:Western blot analysis using HLA-F mAb against human HLA-F (AA: 22-305) recombinant protein. (Expected MW is 35.6 kDa)
Western Blot
Figure 3:Western blot analysis using HLA-F mAb against HEK293-6e (1) and HLA-F (AA: 22-305)-hIgGFc transfected HEK293-6e (2) cell lysate.
Western Blot
Figure 4:Western blot analysis using HLA-F mouse mAb against Mouse Liver (1) tissue lysate.
Flow cytometric
Figure 5:Flow cytometric analysis of Jurkat cells using HLA-F mouse mAb (green) and negative control (red).

HLA-F

貨號 : 32261

規格/濃度 : 1.0mg/ml

價格：

￥ 2557.5

瀏覽量:

1

說明書:

產品規范

Entrez GeneID	3134
別名	HLAF; CDA12; HLA-5.4; HLA-CDA12
克隆編號	3F11F4
分子量	39kDa
同種型	Mouse IgG2b
存放說明	4℃; -20℃ for long term storage
種屬反應性	Human; Mouse;
免疫原	Purified recombinant fragment of human HLA-F (AA: 22-305) expressed in E. Coli.
存儲溶液	Purified antibody in PBS with 0.05% sodium azide

經測試應用

WB	1/500 - 1/2000
FCM	1/200 - 1/400
ELISA	1/10000

描述

This gene belongs to the HLA class I heavy chain paralogues. It encodes a non-classical heavy chain that forms a heterodimer with a beta-2 microglobulin light chain, with the heavy chain anchored in the membrane. Unlike most other HLA heavy chains, this molecule is localized in the endoplasmic reticulum and Golgi apparatus, with a small amount present at the cell surface in some cell types. It contains a divergent peptide-binding groove, and is thought to bind a restricted subset of peptides for immune presentation. This gene exhibits few polymorphisms. Multiple transcript variants encoding different isoforms have been found for this gene. These variants lack a coding exon found in transcripts from other HLA paralogues due to an altered splice acceptor site, resulting in a shorter cytoplasmic domain.

文獻

1.Hum Reprod. 2020 Mar 27;35(3):705-717.
2.Int J Mol Sci. 2019 Nov 8;20(22):5572.

序列

extra(22-305)

圖表

Elisa

Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

Western Blot

Figure 2:Western blot analysis using HLA-F mAb against human HLA-F (AA: 22-305) recombinant protein. (Expected MW is 35.6 kDa)

Western Blot

Figure 3:Western blot analysis using HLA-F mAb against HEK293-6e (1) and HLA-F (AA: 22-305)-hIgGFc transfected HEK293-6e (2) cell lysate.

Western Blot

Figure 4:Western blot analysis using HLA-F mouse mAb against Mouse Liver (1) tissue lysate.

Flow cytometric

Figure 5:Flow cytometric analysis of Jurkat cells using HLA-F mouse mAb (green) and negative control (red).

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